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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Pharmacol Res. 2019 Jan 2;141:331–342. doi: 10.1016/j.phrs.2019.01.002

Fig. 1. Expression of syncytialization-related genes and the BCRP transporter in human placental explants treated with anandamide.

Fig. 1.

A. mRNA expression of BCRP and syncytialization-related genes. qPCR was performed on total RNAs extracted from term placental explants treated with AEA (1 μM) for 24 h as described in the Materials and Methods. Data represent the mean of 3 placentas (3-6 samples from each placenta) ± SE. B. Left. Representative image of protein expression of BCRP in AEA-treated explants. Protein homogenates were prepared from control and AEA (1 μM, 24 h) treated term placental explants and analyzed for expression of BCRP (72 kDa) by Western blotting. β-Actin (42 kDa) was used as a loading control. Right. Densitometry analysis of BCRP Western blots from control and AEA (1 μM, 24 h) treated explants. Data represent the mean of 3 placentas (3-5 samples from each placenta) ± SE. Asterisks (*) represents statistically significant differences (p < 0.05) compared to control explants.