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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Pharmacol Res. 2019 Jan 2;141:331–342. doi: 10.1016/j.phrs.2019.01.002

Fig. 4. AEA is not a substrate or inhibitor of BCRP.

Fig. 4.

A. 3H-Anandamide accumulation in human BeWo trophoblast cells. Untreated BeWo cells were trypsinized and subjected to the 3H-AEA (100 nM) transport assay as described in Materials and Methods. During the uptake and efflux phase, cells were incubated in the presence of Ko143 (1 μM), MM-22 (25 μM), or DMSO. One set of cells was incubated at 4°C for the duration of the assay. Data are presented as mean ± SE (n = 6). B. Competitive inhibition of Hoechst 33342 efflux in BeWo cells. Untreated BeWo cells were trypsinized and subjected to either the Hoechst 33342 (5 μM) cell accumulation assay as described in the Materials and Methods in the presence of vehicle, AEA (10 μM), or Ko143 (1 μM) during the uptake and efflux phase. Data are presented as mean ± SE (n = 3). Asterisks (*) represent statistically significant differences (p < 0.05) compared to control cells.