Figure 3:

Intraperitoneal flavopiridol treatment after injury prevented global metabolomic changes induced by joint injury. All panels show right legs of mice subjected to either injury, injury and flavopiridol (FP), or uninjured controls from naïve mice that received neither injury nor drug, (statistical comparisons #4 and #6, see methods). (A) Unsupervised clustering performed on median values of 426 metabolites common to all experimental groups. The injured + avopiridol group clustered most closely to the naїve uninjured control group. The distribution of injured mice was ~63% further than the distance between the control and avopiridol-treated mice. We identified a cluster of 75 injury-responsive metabolites. (B) Selected metabolites upregulated by injury and rescued by avopiridol treatment. Flavopiridol prevented the injury-induced increases in vitamin D3 and derivatives, acetylcarnitine, and phylloquinone. All p < 0.01 when comparing injured to either controls or injured + avopiridol group using FDR-corrected t-tests for comparisons #4 and #6, with n=6 animals per condition.