(A) Uptake and (B) transfection efficiency of Comb and VIPER polyplexes in HeLa, Jurkat, and primary T cells. Uptake efficiencies were expressed as percentage of YOYO-positive cells. Transfection efficiencies are expressed as percentage of GFP-positive cells. Data are shown as mean ± SD (n=3; 2-way ANOVA with Sidak’s multiple comparisons, *p<0.05, **p<0.01, ****p<0.0001).