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. Author manuscript; available in PMC: 2020 Feb 26.
Published in final edited form as: Biomater Sci. 2019 Feb 26;7(3):789–797. doi: 10.1039/c8bm01262h

Fig. 4.

Fig. 4

IFITM protein expression analysis and polymer transfections of pmaxGFP plasmid in IFITM overexpressing Jurkat T cell lines. (A) Protein expression of individual IFITM 1, 2, and 3 proteins in Jurkat cell lines visualized by western blot with 20 kDa molecular weight marker (MWM). (B) Protein expression of IFITM 1, 2, and 3 in HeLa, parental Jurkat, vector only, and IFITM3 overexpressing Jurkat cells. (C) Transfection efficiency and (D) viability of cationic polymers in Jurkat human T cell lines overexpressing IFITM 1, 2, or 3. Transfection efficiencies are expressed as percentage of GFP-positive cells. Data are shown as mean ± SD (n=3; 2-way ANOVA with Dunnett’s multiple comparisons, ****p<0.0001).