Transfection of Jurkats with 3-MA treatment. (A) Transfection efficiency, (B) viability, (C) transfected cell count, and (D) live cell count of Jurkat cells transfected with Comb at varying 3-methyladenine concentrations. Transfection efficiencies are expressed as percentage of GFP-positive cells. Data are shown as mean ± SD (n=3; 2-way ANOVA with Dunnett’s multiple comparisons, * p<0.05, *** p<0.001, ****p<0.0001).