Table 1.
Primers of foc‐3b used for quantitative reverse transcriptase PCR
| Gene | Accession number | Primer | Position | Sequence(5′‐3′) | AT (°C) | Product size (bp) |
|---|---|---|---|---|---|---|
| fga1 | AB072451.1 | fga1‐f | 557 | GATCGTGGTGTGCAAGAATG | 60 | 180 |
| fga1‐r | 736 | GGAGGACATCCTGGTCGTTA | ||||
| snf1 | AF420488.1 | snf1‐f | 4,107 | TTGTCCACCTCTCCTTCACC | 60 | 177 |
| snf1‐r | 4,283 | GCCTGAAATGGAGCAGAAAG | ||||
| frp1 | AY673970.1 | frp1‐f | 1,798 | TCGTGGCATACTCTCGTCAC | 60 | 180 |
| frp1‐r | 1,977 | CATTAGAAAGGCGAGCTTGG | ||||
| chsV | KC840941.1 | chsV‐f | 3,463 | GGCCAAGACGTTTCCAAGTA | 60 | 180 |
| chsV‐r | 3,642 | CAGGATAGATGCGAGCATGA | ||||
| β‐tubulin | — | Fo‐Tub‐F5 | — | TGTTCGACCCCAAGAACAT | 60 | 158 |
| Fo‐Tub‐R5 | — | GGTCCTCGACCTCCTTCATA |
AT, annealing temperature.