Table 1B.

Measure of the power laser energy (Watt, W) in the experimental set-up.

	Wavelength (nm)	Set-up's energy (laser device) (W)	Output energy (contact) (W)	Coming down energy (air, 3 mm)	Coming down energy (air, 1.5 cm)
AIR (a)
Standard hand-piece	980	1	0.98 ± 0.03	0.87 ± 0.01 W*/**	0.78 ± 0.02 W*/**
Flat-top hand-piece	980	1	1.01 ± 0.02	1.00 ± 0.01 W**	1.02 ± 0.03 W**
	Wavelength (nm)	Set-up's energy (laser device) (W)	Coming down energy (air, 3 mm)	Coming down energy (air, petri, 3 mm)
PETRI DISH (b)
Standard hand-piece	980	1	0.87 ± 0.01 W*/**	0.79 ± 0.02 W+/*/**
Flat-top hand-piece	980	1	1.00 ± 0.01 W**	0.91 ± 0.02 W+/*/**
	Wavelength (nm)	Set-up's energy (laser device) (W)	Coming down energy (air, 3 mm)	Coming down energy (air, αMEM, petri, 3 mm)
αMEM + PETRI DISH (c)
Standard hand-piece	980	1	0.87 ± 0.01 W*/**	0.72 ± 0.02 W+/*/**
Flat-top hand-piece	980	1	1.00 ± 0.01 W**	0.85 ± 0.02 W+/*/**
	Wavelength (nm)	Set-up's energy (laser device) (W)	Coming down energy (air, 3 mm)	Coming down energy (air, αMEM, 3 mm)
ENERGY THAT ARRIVES TO THE CELL MONOLAYER
Standard hand-piece	980	1	0.87 ± 0.01 W*/**	0.80 ± 0.02 W+/*/**
Flat-top hand-piece	980	1	1.00 ± 0.01 W**	0.93 ± 0.02 W+/*/**

Variation of energy depending by the target distance and the features of the material crossed are reported. The laser device set-up was: 1 W, irradiation time of 60 s, in continuous wave (CW) mode.

^*Difference statistically significative respect to the output energy of laser device: p < 0.001.

^**Difference statistically significative between Standard hand-piece vs. Flat-top hand-piece: p < 0.001.

⁺Difference statistically significative respect to the Coming down energy (Air, 3 mm): p < 0.001.