Figure 4.

Profound changes in regulatory gene expression distinguish PU.1-induced lineage deviation from PU.1 effects within the T-cell pathway. Charts show changes in expression of the indicated genes (log₂ Fold Change relative to controls) induced by introduction of PU.1 into post-commitment pro-T cells (DN2b-DN3). Panels compare effects on T-lineage regulatory genes (top) and non-T regulatory and signaling genes (bottom) between cells remaining within the T-lineage pathway (light blue bars) and cells undergoing lineage deviation (dark blue bars). Light, dark red bars show corresponding measured levels of exogenous PU.1 in these samples, as log₂ fold changes over controls, which have downregulated most of their endogenous PU.1 expression at this stage. Results are from Ungerbäck et al. (85).