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. 2019 Feb 20;10:228. doi: 10.3389/fimmu.2019.00228

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Copyright © 2019 Rothenberg, Hosokawa and Ungerbäck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Summary of PU.1 binding features in pro-T cells. (1) PU.1 binding is preferentially retained at high-affinity sites as pro-T cells progressively reduce their PU.1 levels. This feature indicates that PU.1 primarily uses mass action (concentration × affinity) to determine its genomic site choices in these cells. (2) PU.1 works as a positive regulator in pro-T cells primarily by binding and controlling accessibility of sites distal to the transcriptional start sites, not at promoters. (3) The binding profiles of full-length PU.1 (PU1WT) show a tradeoff between binding site affinity and binding site accessibility in chromatin; however, constructs with the PU.1 DNA binding domain but lacking the transactivation domains (PU1 DBD) are poor at engaging sites in closed chromatin no matter how high their potential affinities.