Table 1.
High confidence targets of PU.1 regulation in pro-T cells.
| Consensus PU.1-repressed target genes | Consensus PU.1-activated target genes |
|---|---|
| 2900079G21Rik | 3110043O21Rik |
| Ablim1 | 5430427O19Rik |
| Adamts9 | 9930111J21Rik1 |
| Adora2a | 9930111J21Rik2 |
| Arsi | Abcb1b |
| Bcl2 | Acer3 |
| Ccnd3 | Actn1 |
| Cd247 | Actr2 |
| Cd28 | Acy1 |
| Cdc25b | Adam11 |
| Cecr5 | Adam15 |
| Clec2i | Adap1 |
| Clic5 | Adgre1 |
| Csrnp1 | Adrbk2 |
| Cx3cr1 | Alcam |
| Cxcr5 | Alox5ap |
| Cxxc5 | Anks3 |
| Dgka | Antxr2 |
| E2f2 | Ap1s3 |
| Eng | Apbb1ip |
| Epcam | Aqp9 |
| Fam160a2 | Arhgap6 |
| Gbp10 | Arhgef40 |
| Gbp11 | Atp13a2 |
| Gbp4 | Atp6v0a1 |
| Gbp6 | Avpi1 |
| Gimap1 | B3gnt7 |
| Gimap4 | BC035044 |
| Gimap6 | Bcat2 |
| Gimap8 | Bex6 |
| Gimap9 | Bloc1s2 |
| Hdac4 | Bri3bp |
| Hid1 | Btk |
| Hsd11b1 | Cacnb2 |
| Il12rb1 | Ccdc180 |
| Ilvbl | Ccl9 |
| Irak3 | Ccnd1 |
| Itk | Cd180 |
| Jph2 | Cd300a |
| Lmo7 | Cd300lf |
| Lztfl1 | Cd33 |
| Mbp | Cd34 |
| Mir1903 | Cd44 |
| Neil1 | Cdh1 |
| Nipal1 | Clec10a |
| Pik3ip1 | Col9a3 |
| Pitpnc1 | Coro2a |
| Pitpnm2 | Cotl1 |
| Ppm1h | Creg1 |
| Prf1 | Crtac1 |
| Ptprf | Csf2rb2 |
| Rab27a | Csgalnact2 |
| Rdh10 | Ctbp2 |
| Repin1 | Cyp4f18 |
| Selplg | Dnase2a |
| Sh2d5 | Dock5 |
| Sh3bp5 | Dstyk |
| Slc11a2 | Ebi3 |
| Slc12a7 | Entpd1 |
| Slc27a1 | Erlin1 |
| Sox13 | Erp29 |
| Spata6 | Fam101b |
| Spib | Fam217b |
| Spo11 | Fam49a |
| Spry1 | Fcgr2b |
| Ssbp2 | Ffar2 |
| Sstr2 | Fgd2 |
| Tas1r1 | Fgr |
| Tecpr1 | Fh1 |
| Tlr12 | Fig4 |
| Tmc8 | Flnb |
| Tnfsf11 | Gapt |
| Tox2 | Gfod1 |
| Traf3ip2 | Gm16712 |
| Trat1 | Gm16897 |
| Trp53inp1 | Gm2a |
| Tspan13 | Gng10 |
| Tspan32 | Gng2 |
| Utrn | Gns |
| Wnt5b | Gpx1 |
| Gucy1a3 | |
| Gusb | |
| Haao | |
| Hbb-b1 | |
| Hbb-b2 | |
| Hbb-bs | |
| Hbb-bt | |
| Hck | |
| Hfe | |
| Hpse | |
| Hsd17b6 | |
| Idh2 | |
| Il12rb2 | |
| Il13ra1 | |
| Il1r2 | |
| Il5ra | |
| Inpp5j | |
| Irf5 | |
| Irf6 | |
| Itgad | |
| Itgam | |
| Itgax | |
| Jak2 | |
| Kcnk12 | |
| Kcnk6 | |
| Khdc1a | |
| Khdc1c | |
| Klhl18 | |
| Kmo | |
| Krt80 | |
| Lair1 | |
| Lmo1 | |
| Lpcat2 | |
| Lrba | |
| Lrrc25 | |
| Lrrc75a | |
| Lst1 | |
| Ltb4r1 | |
| Ltbr | |
| Lyn | |
| March1 | |
| Matk | |
| Mb21d1 | |
| Mef2c | |
| Megf8 | |
| Met | |
| Mfsd12 | |
| Myo1f | |
| Naaa | |
| Nccrp1 | |
| Ncf1 | |
| Ncf2 | |
| Ndst1 | |
| Ndufb8 | |
| Nedd9 | |
| Neurl3 | |
| Nfam1 | |
| Nlrc4 | |
| Nlrp10 | |
| Nlrp1b | |
| Nod2 | |
| Npl | |
| Nuak2 | |
| Oas1a | |
| Oas2 | |
| Ogfrl1 | |
| P2ry13 | |
| P2ry14 | |
| Padi2 | |
| Pak1 | |
| Pdxk | |
| Phactr2 | |
| Pik3ap1 | |
| Pik3r6 | |
| Piwil2 | |
| Pla2g4a | |
| Plac8 | |
| Pld4 | |
| Plek | |
| Plxnd1 | |
| Pmvk | |
| Pqlc1 | |
| Prex1 | |
| Prkcd | |
| Prtn3 | |
| Ptpn6 | |
| Ptpre | |
| Rab31 | |
| Ralb | |
| Rcn3 | |
| Relt | |
| Rgs18 | |
| Rnf149 | |
| Rogdi | |
| Rufy1 | |
| Samhd1 | |
| Sema3c | |
| Serpina3g | |
| Sh2b2 | |
| Sh3pxd2a | |
| Siglece | |
| Siglecf | |
| Siglecg | |
| Skap2 | |
| Sla | |
| Slc16a7 | |
| Slc35d3 | |
| Slc8a1 | |
| Snx10 | |
| Sorl1 | |
| Spi1 | |
| Stx7 | |
| Susd3 | |
| Svip | |
| Syk | |
| Tbc1d24 | |
| Tbxas1 | |
| Tdrd7 | |
| Tgm1 | |
| Themis2 | |
| Tlr9 | |
| Tmc5 | |
| Tmem51 | |
| Tmprss3 | |
| Tnni2 | |
| Tor3a | |
| Trim55 | |
| Trmt2a | |
| Trpm2 | |
| Tyrobp | |
| Ufsp2 | |
| Unc93b1 | |
| Vamp8 | |
| Vps18 | |
| Wdfy4 | |
| Ywhag | |
| Zc3h12d | |
| Zfp385a | |
| Zfp52 |
This table lists high-confidence PU.1-regulated genes which show reciprocal responses in the loss of function and gain of function perturbations of PU.1. The lists give the three-way intersection of genes affected in loss of function, and (reciprocally) in gain of function within cells remaining CD25+, and in gain of function within cells becoming CD25− CD44+. Consensus PU.1-repressed target genes: genes with expression that goes down in PU.1-transduced DN2b with or without lineage diversion (CD25+ or CD44+ cells), and also increases in normal DN2a cells after deletion of endogenous PU1. Consensus PU.1-activated target genes: genes with expression that increases in PU.1-transduced DN2b with or without lineage diversion, and also goes down in normal DN2a cells after deletion of endogenous PU.1. Data compiled from Ungerbäck et al. (85).