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. 2019 Feb 26;9:2832. doi: 10.1038/s41598-019-39397-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression patterns of some important miRNAs associated with leaf development. (A) A schematic pathway depicting various miRNAs that regulate the different stages of leaf development from initiation to maturation. Six main developmental processes are highlighted in blue. Corresponding miRNAs and representative leaves in the wild type are listed and shown in each box. (B) The relative expression abundance of several miRNAs in single- and double-mutant plants as detected by small RNA-seq. The numbers in the table refer to the normalized expression of the miRNA, each row indicates the expression abundance of the same miRNA in WT, STTM160, STTM165/166, and STTM160 × 165/166. Data were derived from two independent experiments. (C) The relative expression level of several miRNAs in WT, single- and double-mutant plants determined by qRT-PCR. U6 served as an internal control. Three independent experiments were performed, each with three replicates. Bars show SE. Asterisks in C indicate that values are significantly different at P < 0.01.