Table 3.

H89 inhibitable kinases expressed in PKA-intact mpkCCD cells.

Gene Symbol	Kinase Family	Relative RNA Abundance*	Relative Protein Abundance‡	In vitro Percent Inhibition by H89 (10 µM)†
Prkacb	AGC	0.64	0.525	99
Rps6kb1	AGC	0.26	0.010	97
Akt1	AGC	3.53	0.122	95
Rps6ka5	AGC	0.22	0.028	95
Rock2	AGC	0.95	0.261	94
Pkn2	AGC	1.01	0.051	94
Rps6ka1	AGC	0.56	0.118	92
Akt2	AGC	0.58	0.024	88
Rps6ka3	AGC	0.17	0.170	81
Chek1	CAMK	0.44	0.004	76
Prkaa1	CAMK	0.64	0.779	74
Mark3	CAMK	1.98	0.061	71
Mink1	STE	0.42	0.001	66
Mark2	CAMK	0.74	0.195	65
Prkcz	AGC	0.24	0.005	65
Melk	CAMK	0.40	0§	62
Cdk2	CMGC	1.37	0.921	59
Aurkb	Other	0.72	0§	55
Map2k2	STE	1.38	0.591	54
Camk1	CAMK	0.69	0.000	53
Stk24	STE	1.09	0.507	51

^*RNA abundance normalized by value for PKA catalytic α. From https://hpcwebapps.cit.nih.gov/ESBL/Database/PKA-KO/GeneSymbol.html.

^‡Protein abundance normalized by value for PKA catalytic α. From https://hpcwebapps.cit.nih.gov/ESBL/Database/mpkCCD_Protein_Abundances/.

^†Percent inhibition of purified enzyme. Data from http://www.kinase-screen.mrc.ac.uk/screening-compounds/341068.

^§Upregulated in PKA-null cells.