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. 2019 Feb 26;9:2799. doi: 10.1038/s41598-019-38730-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electrophysiological properties of Purkinje cells from control and PC-mTOR Tg mice. (a) Specimen traces of voltage responses to depolarizing current injection of 0.2 nA (bottom), 0.4 nA (middle), and 0.8 nA (top) recorded in a control Purkinje cell (left), a PC-mTOR Tg Purkinje cell without firing (middle) and a PC-mTOR Tg Purkinje cell generating action potentials (right). Depolarizing currents were applied from −65 mV. (b) Summary of mean spike rates in response to current commands from 0 to 1.0 nA. The firing rate was significantly lower in PC-mTOR Tg than control mice. *p < 0.001 by two-way repeated measures ANOVA; control, n = 30; PC-mTOR Tg, n = 24. (c) Specimen traces of CF-EPSCs in response to paired stimuli with 50 ms interval in a control (upper) and a PC-mTOR Tg Purkinje cell (lower). Holding potential (Vh) was −10 mV. (d) Summary histogram showing the number of discrete CF-EPSC steps in Purkinje cells from control (n = 38 cells from 4 mice) and PC-mTOR Tg mice (n = 22 cells from 3 mice) showed significant difference (*p < 0.001 by Mann-Whitney Rank Sum test). (e) CF-EPSP in a control (upper) and a PC-mTOR Tg Purkinje cell (bottom) elicited by stimulating a single CF that elicited the largest EPSCs in each Purkinje cell. Vh = −65 mV. The histograms represent the numbers of spikes and spikelets (control, n = 17; PC-mTOR Tg, n = 14). The number was significantly smaller in PC-mTOR Tg than control (*p < 0.001 by Mann-Whitney Rank Sum test; control, 2.93 ± 0.20, n = 14; PC-mTOR Tg, 1.06 ± 0.18, n = 17).