Table 1.
MRM parameters for light and SIS peptides.
| S. No. | Name | Sequence | Polarity | Precursor ion (m/z) | Product ion (m/z) | Collision Energy | S-lens Voltage | Retention time (min) |
|---|---|---|---|---|---|---|---|---|
| MRM parameters for light and SIS peptides of S100A8 | ||||||||
| 1 | Peptide I | GNFHAVYR | +ve | 482.24 | 508.16 | 20 | 110 | 7.7 |
| 2 | GNFHAVYR | +ve | 482.24 | 645.46 | 20 | 110 | 7.7 | |
| 3 | GNFHAVYR | +ve | 482.24 | 792.61 | 20 | 110 | 7.7 | |
| 4 | Peptide I* | GNFHAVYR* | +ve | 487.28 | 518.26 | 20 | 95 | 7.7 |
| 5 | Peptide II | MLTELEK | +ve | 432.23 | 518.21 | 15 | 90 | 8.5 |
| 6 | MLTELEK | +ve | 432.23 | 619.35 | 15 | 90 | 8.5 | |
| 7 | MLTELEK | +ve | 432.23 | 732.41 | 15 | 90 | 8.5 | |
| 8 | Peptide II* | MLTELEK* | +ve | 436.4 | 627.36 | 15 | 90 | 8.5 |
| MRM parameters for light and SIS peptides of S100A9 | ||||||||
| 9 | Peptide III | LGHPDTLNQGEFK | +ve | 728.36 | 936.31 | 25 | 148 | 8.2 |
| 10 | LGHPDTLNQGEFK | +ve | 728.36 | 1148.7 | 25 | 148 | 8.2 | |
| 11 | LGHPDTLNQGEFK | +ve | 728.36 | 1310.68 | 25 | 148 | 8.2 | |
| 12 | Peptide III* | LGHPDTLNQGEFK* | +ve | 732.5 | 1156.5 | 25 | 125 | 8.2 |
| 13 | Peptide IV | LTWASHEK | +ve | 486.25 | 571.52 | 20 | 111 | 7.9 |
| 14 | LTWASHEK | +ve | 486.25 | 757.38 | 20 | 111 | 7.9 | |
| 15 | LTWASHEK | +ve | 486.25 | 858.01 | 20 | 111 | 7.9 | |
| 16 | Peptide IV* | LTWASHEK* | +ve | 490.27 | 765.26 | 20 | 91 | 7.9 |
*Indicates the stable-isotope-labelled standard (SIS) peptides; Bold in the peptide amino acid sequence refers to the labelled amino acid residue; Italic figures indicates transition ion used for quantitation.