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. 2019 Feb 26;9:2773. doi: 10.1038/s41598-019-39161-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Verification of isomeric plastome arrangements. (A) Primer pairs designed to amplify specific regions of isomeric arrangements. (B) PCR results generated from 20, 25, 30, and 35 cycles of reactions to determine the major and minor isomeric arrangements in six exemplified Taxus accessions. The gel images were cropped from the full-length gels shown in Fig. S3. (C) Counts of Illumina pair-end reads that support isomeric arrangements. Stacked horizontal bars indicate the relative frequency between arrangements A and B.