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. 2019 Feb 26;9:2765. doi: 10.1038/s41598-019-39504-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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hPSC-derived podocytes exhibit key podocyte phenotypes. IMR90-4 iPSC-derived podocytes were differentiated as illustrated in Fig. 1A. Phase contrast images of IMR90-4 iPSC-derived podocytes were taken at (A) day 6, and (B,C) day 16. (D) Proliferation of cells at different time points was assessed by flow cytometry for Ki67. (E) IMR90-4 iPSC-derived podocytes at day 16 were analyzed with an Albumin Uptake Assay Kit. Alexa Fluor™ 555-labelled albumin is shown in red on a merged DAPI image and the corresponding bright field image is provided on the right. 4 °C was used as a control to prevent endocytosis. Scale bars, 100 µm. Albumin uptake assay was performed three times from three different differentiations.