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. 2019 Feb 26;9:2763. doi: 10.1038/s41598-019-39247-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effective anti-virus activity of AHK after silencing the COI1 gene. (a) Shows the replication level of the NSs gene in the curative effect assay in the TRV-Control and TRV-CoI1 tobacco; (b) shows the replication level of the RdRp gene in the protective effect assay in the TRV-Control and TRV-CoI1 tobacco; (c) shows the transcription level of the RdRp gene in the silenced tobacco and inoculated with TSWV 6 h after different treatments; AHK: AHK treatment; positive: samples only inoculated with TSWV; AHK + JA: silenced tobacco and treatment with AHK and JA; JA: silenced tobacco and treatment with JA; CK: Silenced tobacco, inoculated with TSWV JA. Differences are considered significant at P ≤ 0.05.