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. 2019 Feb 26;9:2753. doi: 10.1038/s41598-019-39073-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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UV irradiation leads to SPB2 mRNA and protein accumulation. The means, standard deviations based on three independent experimental triplicates are indicated in case of (c–e). (a) The numbers of genes showed altered expression following UV irradiation detected by microarray experiment in Hker E6SFM keratinocyte cells. (b) The UV damage induced log₂ expressional changes of the Serpin family members presented in microarray experiment are shown in Hker E6SFM keratinocytes (c), A375 melanoma cells (d) and U2OS osteosarcoma cells (e) by qPCR. Data were normalized to 18 S RNA. (f,g) Western blot detection of changes in SPB2 protein level 2-, 8- and 24 hours following UV irradiation in (f) Hker E6SFM and (g) A375 cells, respectively. H3 was used as loading control. (h) Western blot detection of SPB2 protein level in fractionalized lysates of U2OS cells 2- and 4 hours post-UV irradiation. H3 was used as loading control. (i) Western blot detection of SPB2 protein level in fractionalized lysates of A375 cells 2- and 4-hours post-UV irradiation. H3, Lamin A and Tubulin were used as loading controls.