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. 2019 Feb 26;51(2):23. doi: 10.1038/s12276-019-0220-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — MSCs were stained with β-catenin (red), FZD1 (green) and DKK1 (green) antibodies (green), and the cells were co-stained with DAPI to indicate the nuclei (blue). a–c β-catenin showed obviously weak staining in the ONFH group. Following treatment with 5′-Aza-dC, β-catenin staining was obviously increased but was still weaker than that of the control group. d–f FZD1 showed obviously weak staining in the ONFH group. Following treatment with 5’-Aza-dC, FZD1 staining was obviously increased but was also weaker than that of the control group. g–i Dkk1 had strong staining in the ONFH group. Treatment with 5′-Aza-dC obviously decreased Dkk1 staining. The control group had weak Dkk1 staining (bars 50 μm)