Table 2.

Primers used for real-time PCR

Genes	Sequence (5′→3′)	Product size
Real-time PCR
FZD1-F	CTCGAGGTTTCCTCACTAGACAA	282 bp
FZD1-R	AATGGTTAAACCGCCCTAAATAA
Axin2-F	GAGTGGACTTGTGCCGACTTCA	189 bp
Axin2-R	GGTGGCTGGTGCAAAGACATAG
Cyclin D1-F	CCGTCCATGCGGAAGATC	86 bp
Cyclin D1-R	ATGGCCAGCGGGAAGAC
Runx2-F	AGATGATGACACTGCCACCTCTG	125 bp
Runx2-R	GGGATGAAATGCTTGGGAACTGC
Osteocalcin-F	CACTCCTCGCCCTATTGGC	112 bp
Osteocalcin-R	CCCTCCTGCTTGGACACAAAG
PPARγ-F	CGAGAAGGAGAAGCTGTTGG	122 bp
PPARγ-R	TCAGCGGGAAGGACTTTATGTATG
GAPDH-F	GGCACAGTCAAGGCTGAGAATG	143 bp
GAPDH-R	ATGGTGGTGAAGACGCCAGTA