Figure 3.

Genome-wide GR localisation patterns and underlying TF motif analysis. (a) Glucocorticoid receptor (GR) protein levels in Mo and Mf, treated or not with 1 μM TA for 4 hours. Beta-actin was used as internal loading control antigen. (b) RNA-seq signal at the NR3C1 gene, which codes for GR, in Mo and Mf exposed for four hours to TA. (c) GR ChIP-seq signal intensity in Mo and Mf at 877 high confidence GR ChIP-seq peaks was used to define four sets of GR peaks (MoMf, Mo, Mf and ‘Weak’). Overlap with a previously determined set of Mo and Mf DHS⁴³ is indicated by darker shading. The inset indicates the percentage of peaks that overlap with these DHS. (d) Enriched motifs in the four types of GR-bound regions sorted as a function of the TF superfamily they relate to. Colouring is based on the fractional difference between background and target frequencies. (e) Motif localisation density in the 401 MoMf-GR peaks, depicted as 40 bins of 20 bp.