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. 2019 Feb 26;9:2755. doi: 10.1038/s41598-019-39370-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IL-1β exposure of adult, fetal and ESC-tenocytes results in different gene expression responses. (A) Representative flow cytometry histograms and dot plots of TNMD expression from three biological replicates of (i) adult, (ii) fetal and (iii) ESC-tenocytes cultured in 2D. Blue represents isotype control, green represents TNMD. (B,C) Fold change in gene expression in fetal, adult and ESC-tenocytes following IL-1β exposure for 72 h compared to control cells (fetal, adult or ESC-tenocytes not exposed to IL-1β) on a log scale. Error bars represent the s.e.m. of three independent biological replicates. *p < 0.05 using an unpaired Student’s t-test.