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. 2019 Feb 26;9:2829. doi: 10.1038/s41598-019-39185-3

Table 1.

Unfolding parameters of urea denaturation of the DENV2C protein and its mutants by fluorescence spectroscopy.

WT L50S L54S L81N I88N
U1/2 (M) 5.3 2.9 2.7 1.9 1.6
ΔG°H2O (kcal.mol−1) 11.44 8.59 8.35 1.51 1.38
m 2.08 2.83 3.05 0.52 1.15
Goodness of Fit
R² 0.9998 0.9998 0.9996 0.9994 0.9992