Table 2.
Dissociation constant (Kd) of DENV2C protein and its single mutants to FAM-labeled RNA.
| WT | L50S | L54S | L81N | I88N | |
|---|---|---|---|---|---|
| Kd [nM] | 124.8 | 228.5 | 189.9 | 302.1 | 228.3 |
| 95% Confidence Intervals | |||||
| Kd [nM] | 114.4 to 137.2 | 209.0 to 252.0 | 178.7 to 224.2 | 282.0 to 325.4 | 206.7 to 254.9 |
| Goodness of Fit | |||||
| Degrees of Freedom | 21 | 20 | 22 | 22 | 22 |
| R² | 0,9954 | 0,9955 | 0.9914 | 0.9964 | 0,9923 |
| Absolute Sum of Squares | 0.0001757 | 0.0006295 | 0.001021 | 0.0003745 | 0.0007309 |
| Sy.x | 0.002892 | 0.00561 | 0.006813 | 0.004126 | 0.005764 |
| Number of analyzed points | 24 | 23 | 25 | 25 | 25 |
| Number of points | 28 | 28 | 28 | 28 | 28 |
Non-linear regression analysis with one-phase association equation using the least squares fitting method available in GraphPad Prism 7 software.