Table 2.
Intrinsic excitability of hippocampal granule cells
| Intrinsic excitability | Sham DCX+ |
Sham DCX− |
MCAO DCX+ |
MCAO DCX− |
M-W U test |
M-W U test |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sham DCX+ vs MCAO DCX+ |
Sham DCX− vs MCAO DCX− |
|||||||||||||
| n/N | Median (IQR) | n/N | Median (IQR) | n/N | Median (IQR) | n/N | Median (IQR) | p | U | η2 | p | U | η2 | |
| Max. inward current (nA) | 20/10 | −0.47 (3.13) | 18/9 | −6.61 (4.34) | 43/17 | −2.93 (4.92) | 17/20 | −8.10 (2.60) | 0.019 | 271 | 8.9% | 0.318 | 122 | 3.1% |
| Max. AP rise (V/s) | 30/11 | 10.5 (213.8) | 18/9 | 390.0 (64.7) | 41/1–7 | 186.7 (341.4) | 20/5 | 353.0 (68.2) | 0.004 | 365 | 12.1% | 0.290 | 143 | 3.2% |
| Max. AP decay (V/s) | 30/11 | −6.9 (54.5) | 18/9 | −86.0 (15.0) | 41/17 | −54.3 (61.8) | 20/5 | −81.4 (9.7) | 0.007 | 383 | 10.4% | 0.041 | 110 | 11.3% |
| AP rise/decay ratio | 30/11 | 1.9 (2.2) | 18/9 | 4.3 (1.3) | 41/17 | 3.4 (3.0) | 20/5 | 4.5 (1.5) | 0.013 | 401.5 | 8.8% | 0.613 | 162.5 | 0.7% |
| AP maturation group | 30/11 | Fraction (%) | 18/9 | Fraction (%) | 41/17 | Fraction (%) | 20/5 | Fraction (%) | 0.005 | 398 | 11.0% | 0.988 | 179 | 0.0% |
| Immature AP | 21/30 (70%) | 0/18 (0%) | 16/41 (39%) | 0/20 (0%) | ||||||||||
| Intermediate AP | 5/30 (17%) | 1/18 (6%) | 8/41 (20%) | 1/20 (5%) | ||||||||||
| Mature AP | 4/30 (13%) | 17/18 (94%) | 17/41 (41%) | 19/20 (95%) | ||||||||||
| In cells with mature AP | ||||||||||||||
| AP threshold (mV) | 4/3 | −47.2 (11.8) | 17/8 | −47.2 (8.9) | 17/13 | −46.0 (7.6) | 19/5 | −42.5 (9.3) | 0.965 | 33 | 0.0% | 0.066 | 103 | 9.8% |
| AP amplitude (mV) | 4/3 | 132.2 (18.6) | 17/8 | 140.6 (9.8) | 17/13 | 133.6 (10.9) | 19/5 | 133.6 (10.4) | 0.965 | 33 | 0.0% | 0.087 | 107 | 8.5% |
| Rheobase (pA) | 4/3 | 30 (11) | 17/8 | 50 (23) | 15/12 | 30 (24) | 19/5 | 40 (20) | 0.736 | 26 | 0.9% | 0.208 | 121 | 5.0% |
| Gain (Hz/pA) | 4/3 | 0.39 (0.8) | 17/8 | 0.26 (0.14) | 15/12 | 0.46 (0.48) | 19/5 | 0.24 (0.10) | 0.885 | 28.5 | 0.1% | 0.271 | 126.5 | 3.5% |
M-W, Mann–Whitney; n/N = number of cells/number of mice. The maximal inward current was measured after incremental membrane potential depolarizations in voltage clamp mode. AP maturation was defined as follows: immature APs had maximal rise slopes of ≤100 V/s, intermediate APs had maximal rise slopes >100 V/s and <300 V/s, while mature AP had maximal rise slopes ≥300 V/s. The gain is the mean slope of I–V curves. The statistical unit is n.