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. 2019 Feb 20;13:16. doi: 10.3389/fnana.2019.00016

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Copyright © 2019 Yu, Chang, Chen, Lu, Shy, Chen, Lee and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation and genotyping of forebrain-specific conditional Ctgf knockout mice. The strategy for generating Ctgf knockout (KO) mice (A). A targeting vector carrying loxP-flanked Ctgf (exons 1–5) was designed employing a bacterial artificial chromosome contained mouse genomic DNA encompassing Ctgf of 29.1 kb. PCR products obtained from tail samples for distinguishing floxed (fl) Ctgf allele (B). Genotyping of control (Emx1-Cre; Ctgf^+/+), heterozygous (Emx1-Cre; Ctgf^fl/+) and homozygous (Emx1-Cre; Ctgf^/fl/fl) knockout mice using forebrain samples (C). The outward appearances of control (Emx1-Cre; Ctgf^+/+) and mutant (Emx1-Cre; Ctgf^fl/+, Emx1-Cre; Ctgf^fl/fl) mice (D).