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. 2019 Feb 20;13:16. doi: 10.3389/fnana.2019.00016

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Copyright © 2019 Yu, Chang, Chen, Lu, Shy, Chen, Lee and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphometric analysis of layer VIb neurons in the somatosensory cortex. Golgi-Cox impregnated layer VIb neurons were collected (40 neurons from 6 control mice and 42 neurons from FbCtgf KO mice, respectively) and reconstructed. Scale bar is 20 μm (A). The dendritic features including the numbers of branching nodes (B) and terminals endings (C), highest order (D), and total dendritic length (E) were measured. The complexity and branching pattern of dendrites were estimated using the concentric sphere method (F–H) and the number of dendritic segments (I). The lengths of intermodal segments and terminal segments were also measured (J). Dendritic segments of different orders were obtained and the densities of dendritic spines were quantified. Scale bar is 5 μm (K). Results are means ± SEM (^∗p < 0.05).