FIG. 6.

Chemical probes for S-glutathionylation. (A) Structures of biotinylated glutathione derivatives, BioGSH and BioGEE. (B) Glycine surrogates with bioorthogonal handles for metabolic incorporation. (C) The glycine surrogates can be incorporated into GSH catalyzed by mutated GSs in living cells, which enables proteomic analyses of native glutathionylation. BioGEE, biotinylated glutathione ethyl ester; BioGSH, biotinylated GSH; CuAAC, copper(I)-catalyzed alkyne-azide cycloaddition; GS, glutathione synthetase; GSH, reduced glutathione. Color images are available online.