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. 2019 Jan 21;142(3):542–559. doi: 10.1093/brain/awy346

Figure 3.

Figure 3

Evidence of mitochondrial enrichment of PLPHP in HeLa cells and growth defects in yeast null for the PLPHP ortholog in several energy sources requiring active mitochondrial metabolism. (A) Western blot of wild-type HeLa cells and HeLa cells with HA-tagged mitochondria (HeLa HA-MITO) that were immunoprecipitated for mitochondrial purification, showing PLPHP enrichment in the mitochondrial fraction, other antibodies show minimal contamination from the cytosol or other organelles. (B) Growth curves of wild-type yeast cells and mutant strains on rich oleate medium. The strains shown are: wild-type (WT, BY4742, blue), fox1Δ (green) and ybl036cΔ (purple). (C) Growth of wild-type and mutant cells after 18 h on 20 g/l glucose and non-fermentable carbon sources: rich oleate, 2% ethanol and 2% glycerol medium. Values are expressed as % growth relative to wild-type. The strains shown are: wild-type (BY4742) (blue), fox1Δ (green) and ybl036cΔ (purple). (D) Growth of wild-type cells and mutant cells on 2% ethanol medium. The strains shown are: wild-type (BY4742) (blue), ybl036cΔ + pPROSC1a (human PLPHP under catalase promoter) (orange) or pPROSC2a (human PLPHP under Tef promoter) (red) and ybl036cΔ + empty vector (purple).