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. 2019 Feb 25;3(4):644–657. doi: 10.1182/bloodadvances.2018022756

Figure 1.

Figure 1.

NCoR1 is essential for lymphoid differentiation but dispensable for erythropoiesis. (A) PB cell counts in 8-week-old control mice (n = 6) and Vav-iCre+, NCoR1f/f mice (n = 5) determined using a Hemavet 950FS analyzer (Drew Scientific). (B) Fluorescence-activated cell sorting (FACS) analysis of T cells (CD3+), B cells (B220+), and myeloid cells (CD11b+) in PB samples from control mice (n = 6) and Vav-iCre+, NCoR1f/f mice (n = 5). Representative FACS profiles (upper panel) and the relative frequency (lower left panel) and cell number (lower right panel) are shown. (C) FACS analysis of immature erythrocytes (CD71+Ter119+) in control and Vav-iCre+, NCoR1f/f (n = 6 per group) PB samples. Representative FACS profiles (upper panel) and the relative frequency (lower panel) are shown. FACS analysis of CD71/Ter119 erythroid subsets in spleen (D) and BM (E) from control and Vav-iCre+, NCoR1f/f mice (n = 5 per genotype). Representative FACS profiles (upper panels) and the absolute cell numbers of ProE (CD71highTer119intermediate), EryA (CD71highTer119highFSChigh), EryB (CD71highTer119highFSClow), and EryC (CD71lowTer119highFSClow) (lower panels) are shown. Data are mean ± standard error of the mean from 3 independent experiments. *P < .05, **P < .01, ***P < .001. Hb, hemoglobin; HCT, hematocrit; LY, lymphocyte; MCH, mean corpuscular hemoglobin; MCHC, mean cell hemoglobin concentration; MCV, mean corpuscular volume; MO, monocyte; MPV, mean platelet volume; NE, neutrophil; RDW, red cell distribution width.