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. 2019 Feb 26;2(2):e201800222. doi: 10.26508/lsa.201800222

Figure 2. FUS relocalises to nucleoli in response to TOP1-induced DNA breakage.

Figure 2.

(A) GFP-FUS fluorescence was detected in untreated HeLa cells and in HeLa cells following treatment with the TOP1 poison CPT (4 μm) for 45 min or 30 min after IR (2 Gy). Scale bars, 10 μm. (B) GFP-FUS fluorescence was detected in HeLa cells treated with DMSO vehicle or with 4 μm of CPT for 1 h. Anti-fibrillarin immunostaining was used to stain nucleoli. Numbers are the mean percentage (±SD) of GFP-positive cells with GFP-FUS nucleolar localization in three independent experiments (50 cells per experiment). Scale bars, 10 μm. (C, D) Endogenous FUS was detected by indirect immunofluorescence in A549 cells (C) and primary human fibroblasts (D) treated with DMSO vehicle or 4 μM CPT for 45 min. Scale bars, 5 μm.