Figure S2. GFP-tagged FUS relocalisation to nucleoli is not affected in human spinal motor neurons by ALS-associated mutation, following CPT treatment.
(A) The GFP-tagged ALS-related mutant protein FUSP525L-GFP (P525L-GFP) was detected by direct fluorescence in human spinal motor neurons after treatment with DMSO vehicle or 4 μM CPT for 1 h. Fibrillarin and MAP2 were detected by indirect immunofluorescence to stain nucleoli and neurons, respectively. Numbers are the mean (±SD) of the percentage of MAP2-positive cells with P525L-GFP nucleolar localization from three independent experiments (50 cells/experiment). Scale bar, 20 μm. Zoomed areas are shown (right). (B) FUSP525L-GFP was detected as above but with co-staining for choline acetyltransferase (ChAT) to confirm the identity of motor neurons. Numbers are the percentage of ChAT-positive cells with P525L-GFP nucleolar localization (n = 100 cells). Scale bar, 10 μm.