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. 2019 Jan 3;31(1):37–51. doi: 10.1105/tpc.18.00721

Figure 4.

Figure 4.

HOS15 Associates with LUX.

(A) HOS15 interacts with LUX in a yeast two-hybrid assay. Shown are the results of a yeast two-hybrid assay using HOS15 or ELF3 as prey and LUX as bait. Empty vector was used as a negative control. Growth on SD-TLH medium indicates a positive interaction. ELF3, which interacts with LUX, was used as a positive control.

(B) BiFC analysis of proteins transiently expressed in Nicotiana benthamiana leaves. VN and VC indicate the N and C termini, respectively, of Venus (eYFP). Fluorescence (left panels) was detected by confocal microscopy. Right panels are overlay of fluorescence and differential interference contest images. Bars = 100 μm.

(C) and (D) HOS15 interacts with LUX. Total protein extracts from 10-d-old plants were immunoprecipitated with α-HOS15 antibodies and resolved by SDS-PAGE. Immunoblots were probed with α-HOS15, or with α-LUX antibodies to detect LUX. Wild type and hos15-2 plants used for (D) were cross-linked in 1% formaldehyde before sampling. *Nonspecific bands.