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. 2018 Dec 20;31(1):52–67. doi: 10.1105/tpc.18.00518

Figure 1.

Figure 1.

Selection of Time Points for Transcriptional Analysis of M0171>>bdl Embryos.

(A) to (D) Embryos from crosses between M0171 and wild type [(A) and (C)] or M0171 and UAS-bdl [(B) and (D)] prepared 72 h [(A) and (B)] or 96 h after pollination [(C) and (D)]. GFP expression reflects the activity of the M0171 enhancer trap, which drives the expression of GAL4:VP16, which in turn activates the linked UAS-erGFP gene. Images show overlay of GFP and cell wall (counterstained with Renaissance RS2200-magenta) signals. Scale bar represents 10 μm in all panels. Arrowheads in (B) and (D) indicate aberrant cell divisions in suspensor cells.

(E) UpSet plot showing overlap between differentially expressed genes (up- or downregulated relative to wild type) in M0171>>bdl embryos at 72 h and 96 h after pollination. Numbers indicate the number of common genes in each comparison.