Figure 8.

bHLH49 Mediates Postembryonic Auxin-Dependent Growth.

(A) Root length of wild type (Col-0), pRPS5A-bHLH49, bhlh49-1, and bhlh49-2 seedlings upon treatment with 10 nM, 20 nM, 30 nM, and 40 nM 2,4-D, compared with untreated control (t test: *P < 0.05; **P < 0.001). Error bars indicate sd in three separate experiments where at least 30 and up to 50 seedlings per genotype were used for the measurement.

(B) to (E) Expression of DR5-n3GFP in wild-type [(B) and (D)] and pRPS5A-bHLH49 [(C) and (E)] globular stage embryos [(B) and (C)] and roots [(D) and (E)]. Magenta counterstaining in (B) and (C) is Renaissance RS2200 and in (D) and (E) it is Propidium iodide. Scale bar = 10 μm in all panels.