Figure 2.

PAPST2 and PAPST1 Mediate Uptake of [α³²P]ATP into Differently Loaded Liposomes.

Import of 50 µM [α³²P]-ATP into PAPST2 (dark gray bars) or PAPST1 (light gray bars; data from Gigolashvili et al., 2012) liposomes loaded with 5 mM of the indicated substrates or into liposomes containing APS. Uptake was stopped at 2.5 min. The data represent net values (minus control values, nonloaded proteoliposomes). Homo-exchange (ATP/ATP transport) was set to 100% and transport into the remaining liposomes was calculated accordingly. Data are the mean of three independent experiments; standard errors are displayed. Asterisks indicate significant difference (Student´s two-tailed t test, P < 0.01).