Figure 7.

The fry1 Phenotype Is Alleviated by the Absence of PAPST1 and Worsened by the Absence of PAPST2.

Phenotypes of fry1 papst1 and the fry1 papst2 double mutant lines compared with the wild-type plants and the fry1 mutant. Plants were grown in soil under short-day conditions.

(A) Adult plants (age: 5 weeks). Bar = 2 cm.

(B) Young seedlings (age: 1 week). Arrows in fry1 papst2 indicate bleaching along the veins of cotyledons. Note fry1 has only slightly more pale areas in cotyledons than the wild-type (Col-0). Bar = 2 mm.

(C) PAP levels in mutants with impaired PAPS and PAP transport. PAP levels in papst2, papst1, fry1 papst2, and fry1 papst1 mutants compared with those of the wild type and fry1. Results represent the means of two independent cultivations and eight biological replicates; and results were analyzed via ANOVA and Tukey’s test (Supplemental Data Set). Error bars indicate the ±sd of the mean. Different letters indicate significant differences at P < 0.05. Plants were grown in soil under short-day conditions for five weeks. FW = fresh weight.