Table 3.

Effects of dermal exposure to DDAB on DLN cell number and lymphocyte sub-populations in mice after 14 days exposure.

DDAB (%)
Parameter	0	0.0625	0.125	0.25
DLN number (×106)	0.348 ± 0.03	0.69 ± 0.07*	1.33 ± 0.10**	1.97 ± 0.09**
B-cell number(×106)	0.79 ± 0.11	1.48 ± 0.17	3.70 ± 0.36**	6.70 ± 0.48**
% of DLN	22.56 ± 1.32	21.38 ± 0.70	27.70 ± 0.65**	33.86 ± 1.07**
CD4+T-cell number(×106)	1.69 ± 0.14	3.52 ± 0.36**	6.14 ± 0.45**	8.00 ± 0.28**
% of DLN	48.98 ± 1.69	51.14 ± 0.92	46.30 ± 0.59	40.74 ± 0.86**
CD8+T-cells(×106)	0.54 ± 0.06	1.15 ± 0.11**	2.14 ± 0.12**	3.24 ± 0.16**
% of DLN	15.64 ± 0.56	16.80 ± 0.51	16.22 ± 0.47	16.46 ± 0.33*
Dendritic Cells(×105)	0.43 ± 0.03	0.78 ± 0.16	1.62 ± 0.18**	2.09 ± 0.19**
% of DLN	1.28 ± 0.11	1.10 ± 0.10	1.22 ± 0.08	1.08 ± 0.08

Mice were dermally exposed to vehicle (acetone) or different concentrations of DDAB for 14 consecutive days. The mice were euthanized six days after the final exposure, DLN were removed, and total cells counted. Numbers and frequency of B- and T-cells, and subsets of T-cells (CD4⁺ and CD8⁺), and dendritic cells were enumerated. Values represent the means (±SE) for each group. Significantly different from acetone controls at

^*p < 0.05

^**p < 0.01.