Fig. 1.

a Immunofluorescent stainings (magnification × 400) of isolated PASMCs derived from WT and SmPparγ^−/− mice. Cells from SmPparγ^−/− mice displayed lack of PPARγ immunoreactivity. b Immunoblotting analyses of isolated aortae from WT and SmPparγ^−/− mice also demonstrates marked reduction in PPARγ expression in vasculature from SmPparγ^−/− animals. c Weight gain curve of WT and SmPparγ^−/− mice on low fat diet (LFD) or high fat diet (HFD). Significant differences (p < 0.05) were detectable between LFD- and HFD-fed mice, regardless of the genotype, starting after 2 weeks of feeding (n = 10–17 per group). For better visualization of weight curves, significances between LFD and HFD of the same genotypes are not depicted