Fig. 5.

Effect of genotype and diet on pulmonary vascular remodelling (arteries sized 20–70 μm) in monitored study groups: a Representative pictures from immunohistochemical stainings of pulmonary tissues in all analyzed animal groups using α-smooth muscle actin antibodies for visualization of vascular muscularization. b Quantification of vascular muscularization was performed in lung arteries. Shown are the ratios of non- and muscularized pulmonary arteries (n = 23–27 arteries / N = 5 animals). There was a borderline effect (p = 0.06) of genotype without interaction with diet (a two-factor linear model with interaction and a non-parametric Kruskal-Wallis test was performed). c Shown are quantifications of lung immunohistochemical stainings with antibodies against osteopontin for detection of extracellular matrix remodelling in each study group (n = 5). A univariate general two factor model with interactions was used for testing. A logarithmic transformation was used to avoid violation of homodekasticity. Additional non-parametric tests (Kruskal-Wallis, Jonckheere-Terpstra) were performed. The p-values were significant for genotype (p < 0.001) and diet (p = 0.021) with significant interaction of genotype with diet (p = 0.001)