Figure 2.

Characterization of Enteroid Polarity Reversal

(A) Enteroids were analyzed using confocal microscopy and quantified for percentage of basal-out, apical-out, or mixed polarity enteroids; n = 3 experiments.

(B) Quantification of basal-out, apical-out, or mixed polarity enteroids in suspension culture with soluble BME; n = 3 experiments.

(C) BME-embedded enteroids were incubated in media alone or with β1-integrin function-blocking antibody or a control antibody for 1 day; n = 3 experiments. For (A)–(C), data represented are the means of each category with SD.

(D) Time-lapse DIC microscopy of immobilized apical-out (top) or BME-embedded basal-out (bottom) enteroids as shown in Video S1.

(E) Confocal microscopy of enteroids from time-lapse experiment. Nuclei in blue, ZO-1 in green, and β-catenin in red are shown. Scale bars are 20 μm.

(F) Confocal microscopy of suspended enteroids at different stages of polarity reversal. Nuclei in blue, ZO-1 in green, and β-catenin in red are shown. Scale bars are 10 μm.

See also Figures S3 and S4 and Video S1.