Table 5.
Genotypic characterization of NDM-positive isolates transferring only blaNDM gene
| Strain no. | Organism | NDM | PMQR | Other Resistance genes | MEM MIC | CIP MIC | Plasmid size in kb (approximately) | Plasmid type (Inc) | Plasmid addiction system | Integron |
|---|---|---|---|---|---|---|---|---|---|---|
| EN5134 | Escherichia coli | bla NDM-15 | aac(6′)-Ib-cr |
bla
CTX-M, TEM, OXA
rmtB |
24 | > 32 | 225, 158,4 | FIA, FII, I1A | PemK, CcdAB, Hok-Sok, PndCA | intI1 |
| EN5134.T1 | E. coli J53 Fox-azide | bla NDM-15 | – | bla TEM, rmtB | 2 | 0.012 | 121 | FII | – | intI1 |
| EN5141 | Escherichia coli | bla NDM-1 | aac(6′)-Ib-cr | bla CTX-M, TEM, OXA, armA | 4 | > 32 | 246, 134, 6 | FIB, HIB-M, FII | PemK, Hok-Sok | – |
| EN5141.T1 | E. coli J53 Fox-azide | bla NDM-1 | – | bla TEM, | 1.5 | 0.008 | 246, 6 | HIB-M | – | – |
| EN5143 | Escherichia coli | bla NDM-1 | – |
bla
TEM
rmtB |
4 | > 32 | 105,56, 9 | FIA, I1γ, FII, I1a | PndC/A | intI1 |
| EN5143.T1 | E. coli J53 Fox-azide | bla NDM-1 | – |
bla
TEM
rmtB |
1.5 | 0.008 | 105 | FIA, FII | – | intI1 |
| EN5169 | Escherichia coli | bla NDM-7 | – |
bla
TEM
rmtB |
> 32 | > 32 | 200, 5, 2 | I1A, FIA, FIB, FIIS, R, FII | PndCA, Hok-Sok, ccdAB, SrnBC, PemK | - |
| EN5169.T1 | E. coli J53 Fox-azide | bla NDM-7 | – | bla TEM, | 1 | 0.012 | 200,5 | I1A, | Pnd C/A | - |
| EN5177 | Escherichia coli | bla NDM-5 | – |
bla
TEM
rmtB |
4 | > 32 | 163,126,55,5, 2 | FIB, FIIS | PemK | IntI1 |
| EN5177.T1 | E. coli J53 Fox-azide | bla NDM-5 | – |
bla
TEM
rmtB |
1 | 0.012 | 163,126 | FIB, FIIS | – | IntI1 |
| EN5114 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr |
bla
CTX-M, SHV, OXA
armA |
1.5 | 1.5 | 248 | HIB-M | PemK | intI1 |
| EN5114.T1 | E. coli J53 Fox-azide | bla NDM-1 | – |
bla
CTX-M
armA |
1.5 | 0.012 | 248,180 | HIB-M | – | - |
| EN5117 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr |
bla
CTX-M
armA |
1.5 | 1.5 | 248 | HIB-M | PemK | intI1 |
| EN5117.T1 | E. coli J53 Fox-azide | bla NDM-1 | – |
bla
CTX-M, SHV, OXA
armA |
1 | 0.016 | 248 | HIB-M | – | - |
| EN5123 | Klebsiella pneumoniae | bla NDM-1 | – | bla CTX-M, aac(6′)-Ib | 3 | 0.094 | 210, 20,7,5 | FIA, R, FIIK | – | intI1 |
| EN5123.T1 | E. coli J53 Fox-azide | bla NDM-1 | – | bla CTX-M, aac(6′)-Ib | 1 | 0.008 | 210,5 | FIIK | – | - |
| EN5129 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr,qnrB | bla CTX-M, aac(6′)-Ib | 2 | 12 | 263,230,15.6,6.7, | FIIK, R | – | intI1 |
| EN5129.T1F-A | E. coli DH10B | - | aac(6′)-Ib-cr,qnrB | bla CTX-M | 0.023 | 0.19 | 230 | FIIK | – | - |
| EN5130 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr | bla CTX-M, OXA | 8 | > 32 | 270,205,29,13, 8, 7, 6,5, 3, 2 | FIIK, FIA, X2 | – | intI1 |
| EN5130.T1F | E. coli DH10B | bla NDM-1 | – |
bla
CTX-M, OXA
- |
2 | < 0.002 | 270, 205, 29, 13, 8, 7,5, 3, 2 | – | – | - |
| EN5135 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr,qnrB | bla CTX-M, TEM, SHV, OXA | 1.5 | 16 | 230, 5, 4, 2, 0.1 | FIIK, HIB-M | VagCD, | intI1 |
| EN5135.T1 | E. coli J53 Fox-azide | bla NDM-1 | – | bla CTX-M | 1.5 | 0.004 | 230 | HIB-M | – | – |
| EN5136 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr,qnrB | bla CTX-M, TEM, OXA | 3 | 4 | 340, 212, 9, 6, 4 | FIIK, HIB-M | PemK, CcdAB, VagCD, Hok-Sok | intI1 |
| EN5136.T1 | E. coli J53 Fox-azide | bla NDM-1 | – | bla CTX-M, TEM | 0.75 | 0.012 | 340 | HIB-M | – | intI1 |
| EN5137 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr,qnrB | bla CTX-M, TEM, OXA | 1.5 | 32 | 310,162, 8, 6, 4 | FIIK, HIB-M | PemK, CcdAB, VagCD, Hok-Sok | intI1 |
| EN5137.T1 | E. coli J53 Fox-azide | bla NDM-1 | – | bla CTX-M, TEM | 0.75 | 0.012 | 310 | HIB-M | – | - |
| EN5139 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr,qnrB | bla CTX-M, TEM, OXA | 1 | 16 | 250,112,5, 4 | FIIK, HIB-M | PemK, VagCD | intI1 |
| EN5139.T1 | E. coli J53 Fox-azide | bla NDM-1 | – | bla CTX-M, TEM, | 1.5 | 0.23 | 250 | HIB-M | – | - |
| EN5142 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr,qnrB |
bla
CTX-M, TEM, OXA
rmtC |
8 | 2 | 248,104 | FIIK, FIB-M | – | intI1 |
| EN5142.T1 | E. coli J53 Fox-azide | bla NDM-1 | – | - rmtC |
1.5 | 0.008 | 248, 104 | – | – | intI1 |
| EN5144 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr,qnrB |
bla
CTX-M, SHV, OXA
armA |
2 | 6 | 227, 52, 5, 4 | HIB-M | – | - |
| EN5144.T1 | E. coli J53 Fox-azide | bla NDM-1 | – |
bla
CTX-M, SHV, OXA
armA |
0.5 | 0.016 | 227 | HIB-M | – | - |
| EN5146 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr | bla CTX-M, rmtC, aac(6′)-Ib | 10 | > 32 | 192,164, 7 | FIA, A/C, FIIK | – | - |
| EN5146.T1F | E. coli DH10B | bla NDM-1 | – | bla CTX-M, aac(6′)-Ib | 6 | < 0.002 | 164 | A/C | – | - |
| EN5154 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr |
bla
CTX-M
armA |
8 | > 32 | 241, 201, 34, 29,8, 6, 4,3 | FIIS, R, FIIK | PemK, VagCD | IntI1 |
| EN5154.T1F | E. coli DH10B | bla NDM-1 | – | bla CTX-M, | 6 | 0.012 | 240, 4 | FIIS | – | IntI1 |
| EN5175 | Klebsiella pneumoniae | bla NDM-1 | aac(6′)-Ib-cr, qnrB |
bla
CTX-M, TEM, OXA
armA |
3 | 4 | 123, 88,7,5,3 | N, FIIK, HIB-M | PemK, VagCD | IntI1 |
| EN5175.T1 | E. coli J53 Fox-azide | bla NDM-1 | – |
bla
CTX-M, SHV, OXA
armA |
1.5 | 0.012 | 123 | HIB-M | - | - |
| EN5175.T2 | E. coli J53 Cip-Azide | – | qnrB | armA | 0.023 | 0.125 | 96, 50 | N, FIIK | VagCD | - |
| EN5180 | Klebsiella pneumoniae | bla NDM-1 | – |
armA,rmtC, bla
TEM
aac(6′)-Ib |
32 | > 32 | 293,208,5,4 | A/C, FIIK, FIB-M, HIB-M | VagCD | IntI1 |
| EN5180.T1 | E. coli J53 Fox-azide | bla NDM-1 | – |
bla
TEM
rmtC |
1.5 | 0.016 | 208 | A/C | IntI1 | |
| EN5186 | Klebsiella pneumoniae | bla NDM-1 | qnrB | armA, rmtC | 6 | > 32 | 235,188,47,5, 3, | FII | – | IntI1,IntI2 |
| EN5186.T1 | E. coli J53 Fox-azide | bla NDM-1 | – |
bla
CTX-M, TEM
rmtC |
1.5 | 0.008 | 188 | - | - | IntI1 |
| EN5131 | Enterobacter aerogenosa | bla NDM-1 | aac(6′)-Ib-cr,qnrB | bla CTX-M, OXA, rmtC | 4 | 4 | 205,27,17,7,5 | – | ccdA/B, hok -sok | intI1 |
| EN5131.T1 | E. coli DH10B | bla NDM-1 | – | bla CTX-M, rmtC | 2 | < 0.002 | 205,27,7 | – | – |
MEM: meropenem, CIP: ciprofloxacin, (-) : absent or untypable (in case of plasmid types), E. coli J53 Fox-azide or ‘.T1’ : transconjugants selected in cefoxitin (10μg/ml)-sodium azide (100μg/ml), E. coli DH10B or ‘.TF’: Transformants selected in cefoxitin (5μg/ml) , ‘.TF-A : Transformants selected in ampicillin (50μg/ml), E. coli J53 Cip-Azide or ‘.T2’ : transconjugants selected in ciprofloxacin (0.06 μg/ml)-sodium azide (100 μg/ml)