Fig. 2.

TRIM29 promotes the migration and invasion of CRC cells in vitro. a, b TRIM29 mRNA and protein levels in six CRC cell lines were examined by qRT-PCR and Western blotting analysis. β-actin was used as an internal control. c, d The effects of TRIM29 knockdown and overexpression were confirmed by qRT-PCR and Western blotting. β-actin was used as an internal control(***P < 0.001). e, f Modified SW620 and RKO cells were subjected to scratch wound-healing assay to examine the migration effect of TRIM29. The wound space was photographed at 0, 9, and 18 h. The cell migration ability was evaluated by measuring the distance between the advancing margins of cells in five microscopic fields at each time point. All of the data are presented as the mean ± SEM. from three independent experiments (***P < 0.001). g, h The migration and invasion assays showed different cell motilities in modified SW620 and RKO cells. Knockdown of TRIM29 clearly inhibited the migration and invasion of SW620 cells. Conversely, overexpression of TRIM29 promoted the migration and invasion of RKO cells. All of the data are presented as the mean ± SEM. from three independent experiments (***P < 0.001)