Fig. 4.

TRIM29 facilitates EMT in CRC cells. a, b The mRNA and protein expression levels of four EMT markers (E-cadherin, N-cadherin, Vimentin, and Slug) in modified SW620 and RKO cells were assayed by qRT-PCR and Western blotting. β-actin was used as an internal control. The data are presented as the mean ± SEM. from three independent experiments (**P < 0.01, ***P < 0.001). c Immunofluorescence assays were used to compare the protein expression levels of E-cadherin, N-cadherin, Vimentin, and Slug in modified SW620 and RKO cells. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) in blue, and targeted proteins were stained in red. d Morphological changes in modified SW620 and RKO cells