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. 2019 Feb 27;7:58. doi: 10.1186/s40425-019-0536-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Fluorescence emission of PpIX from cell subsets in the tumor microenvironment. a Representative flow cytometry plot and (b) cumulative analysis of 5-ALA fluorescence emission by CD45⁻ cells (pink), MG (blue) and BMDM (green) (n = 15 core samples, n = 21 intense fluorescence samples and n = 13 margin samples). c t-SNE analysis of live cells infiltrating the intense fluorescence layer (n = 11 samples) of GBM patients. Combined analysis on the following parameters: CD45, CD33, HLA-DR, CD49D, PPIX. In the two main clusters obtained after t-SNE analysis (blue and green populations), the expression of the single markers was analyzed (blue and green histograms). Comparison by Mann-Whitney test, **p < 0.01