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. 2019 Feb 26;20:93. doi: 10.1186/s12891-019-2469-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Tracer diffusion coefficients of FITC-dextran (2000 kDa) through 1500 kDa HA solutions ±450 μg/ml BSA (∆ for HA + BSA, ● for HA) (a), 500 kDa HA solutions ±450 μg/ml BSA (b); 1500 kDa HA solutions ±45 μg/ml BSA (c), 500 kDa HA solutions ±45 μg/ml BSA (d). Data points are fit to the universal scaling equation (dashed lines = HA + BSA, solid lines = HA). At 450 μg/ml BSA, in both 1500 and 500 kDa HA (a, b) the diffusion of the tracer was significantly affected by HA concentration (both p < 0.01) but not the presence of BSA (p = 0.39 and 0.11 respectively), with no interaction effect (p = 0.49 and 1.0 respectively). At 45 μg/ml BSA, in both 1500 and 500 kDa HA (c, d) the diffusion of the tracer through 1500 kDa HA was significantly affected by HA concentration (both p < 0.01) but not the presence of BSA (p = 0.95 and 0.73 respectively), with no interaction effect (p = 0.99 and 0.372, respectively)