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. 2019 Feb 20;10:278. doi: 10.3389/fmicb.2019.00278

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Copyright © 2019 Qin, Zhao, Yu, Liu, Chu, Tian and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the cadmium resistance of different mutant strains. (A,B) Cadmium resistance of htpX-overexpressing strains, with BL21-pUC19 as a negative control; in BL21-pUC19-htpX (V16A), the htpX gene was overexpressed, and the V16 residue of HtpX was mutated from Val (V) to Ala (A). (C,D) Cadmium resistance of gor-overexpressing strains; in BL21-pUC19-gor (G249A), the gor gene was overexpressed, and the G249 residue of Gor was mutated from G (Gly) to Ala (A). 1.0 and 1.2 mM represent the cadmium MIC values.