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. 2019 Feb 19;25:1323–1335. doi: 10.12659/MSM.913746

Figure 2.

Figure 2

CCND2 and P2RY1 are direct targets of miR-34b-3p in BCa cells. Levels of CCND2 and P2RY1 mRNA (A) and protein (B) in miR-34b-3p mimic (3PM)-transfected 5637 cells and the miR-34b-3p antagomiR (3PA)-transfected EJ cells versus negative control (NC) cells, as determined by real-time PCR or western blot analyses. Vector information of CS-HmiT021075-MT01 used in the untranslated region (UTR) luciferase activities (C). Relative luciferase activities (fold) of the reporter with the wild-type (WT) CCND2-UTR/P2RY1-UTR or without the UTR (Vec) were determined in the BCa cells transfected with the 3PM (in 5637), 3PA (in EJ) or Mock (Vec) (D) sequences. The Renilla luciferase activity of a co-transfected control plasmid was used as a control for the transfection efficiency. Representative results from 3 independent experiments are shown. * P value <0.05, ** P value <0.01 by Student’s t-test. CCND2 – G1/S-specific cyclin-D2; P2RY1 – purinergic receptor P2Y1; BCa – bladder cancer; PCR – polymerase chain reaction.