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. Author manuscript; available in PMC: 2019 Jun 12.
Published in final edited form as: Nature. 2018 Dec 12;565(7739):318–323. doi: 10.1038/s41586-018-0804-9

Extended Data Figure 4. Characterization of the CD4-gp120-CCR5 complex by EM.

Extended Data Figure 4.

(a) Representative image of the CD4-gp120-CCR5 complex in negative stain. The experiment was repeated independently at least 4 times with similar results. (b) 2D averages of the negatively stained CD4-gp120-CCR5 complex. The box size of 2D averages is ~330Å. (c) 3D reconstruction of the negatively stained CD4-gp120-CCR5 complex, fitted with a gp120 structure containing an extended V3 loop (pdb ID: 2QAD; ref20), 4D CD4 (pdb ID: 1WIO) and CCR5 (pdb ID: 4MBS). (d) A representative cryo-EM image of the 4D CD4-gp120-CCR5 complex. The scale bar represents 25 nm. Five independent large data sets were collected with similar results. (e) 2D averages of the cryo-EM particle images show secondary structural features for both gp120 and CCR5.